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Cells were mock treated or exposed to 1 mM formaldehyde for 1 h. Following drug removal, cells were allowed to recover as indicated and labelled with 1 mM 4SU (Glentham Life Sciences) during the last 30 min. Immediately after 4SU labelling, cells were washed with PBS and lysed ...
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b Volcano plot depicting the statistical differences of the MS analysis on GFP-UVSSA pull-down in mock-treated and UV-irradiated samples. The enrichment (log2) is plotted on the x-axis and the significance (t-test -log p value) is plotted on the y-axis. All significantly UV-induced ...
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always keep stocked in my fridge. The best part of any soup or stew, in my most humble opinion, is the garnish. A squeeze of lime, sour cream, cilantro, and shredded Monterrey Jack (“Modern Jack” to my kids, in their mind, a perfect accompaniment to chips and “Mock ‘n Mole”....
a, Top: scheme of experiment. Bottom: relative GFP–RPB1 protein levels measured by flow cytometry in the presence of 100 µM cycloheximide after a pulse of 1 mM FA for 30 min. RFI of GFP were normalized to mock-treated levels and set to 1. Bars represent the mean fluorescence...