A Duolink® proximity ligation assay kit (Merck, DUO92102, Darmstadt, Germany) was used to perform the proximity ligation assay by following the manufacturer’s instructions. Anti-USP53 Rabbit polyclonal Ab (Thermo Fisher, PA5-57424) was conjugated with a PLUS nucleotide probe, while anti-CY...
In brief, 3 × 104 cells in 200 μl of FBS-free medium were seeded in the wells of 8 μm-pore Boyden chamber (Millipore Darmstadt, Germany) coated with (for invasion) or without (for migration) 1:7.5 diluted Matrigel (Sigma, St. Louis, MO). Cells were maintained in ...
(#81201, Ibidi, Martinsried, Germany), fixed for 20 min in 4% paraformaldehyde in PBS, permeabilized for 15 min in 0.25% Triton-X-100 in phosphate-buffered saline (PBS), blocked with 5% normal goat serum (Merck Millipore, Darmstadt, Germany) in PBS and stained cell–cell junctions,...
the two groups of 35 elementary students in total were found to have progressed in the same way regarding mastery of programming concepts. However, the group taught using CS Unplugged material showed higher self-efficacy and used a wider vocabulary of Scratch blocks. Similarly, Gaio [49] conclude...
For testing matrix effects, sodium chloride from AppliChem (for analysis, AppliChem GmbH, Darmstadt, Germany), aluminum nitrate from Merck (Certipur® grade purchased from Merck KGaA, Darmstadt, Germany), and sodium persulfate from Honeywell (≥99.0 %, Honeywell, Charlotte, North Carolina, U.S...
Then the slides were immersed in xylene for 10 minutes, and then dried and covered with Enthelan R (Merck, Darmstadt, Germany) then covered by cover glasses. These slides were observed using Olympus BX53 microscope (Olympus Corporation, Tokyo, Japan) with CellsSens Standart software version 1.5...
Four new mixed-valent chain alkali metal ( A ) sulfido ferrates of the general structure family A1+xFexIIFe1−xIIIS2${A}_{1+x}\left[{\text{Fe}}_{x}^{\text{II}}{\text{Fe}}_{1-x}^{\text{III}}{\text{S}}_{2}\right]$ were synthesized in the form of tiny gre
All reagents used for the synthesis of antibiotic-loaded composite spheres were purchased from Sigma-Aldrich (Merck Group, Darmstadt, Germany), namely poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid)—P(3HB-3HV) with 8 mol% polyhydroxyvalerate content, high purity chitosan (CS) with ≤40 ...
Materials The trihexyl(tetradecyl)phosphonium chloride (Cyphos IL-101) was purchased from Sigma-Aldrich (Oakville, ON, Canada), and the Florisil® support was supplied by Merck (Darmstadt, Germany). The Cs+ aqueous solutions were prepared by adequate dilution of stock solution of 1,000 mg/L ...
Scenario #1: 5 Scenario #2: 2 Source TUD Programming Contest 2002, Darmstadt, Germany 二维数组a[i][j]表示尾数为i(i = 0 or i = 1),长度为j的不同序列的个数。显然有 a[0][1] = a[1][1] = 0; a[0][j] = a[0][j-1] + a[1][j-1]; ...