Crystallization and structure determination of Staphylococcus aureus NAD synthetaseAn unliganded form of NAD synthetase (NadE) has been crystallized, and the three-dimensional x-ray crystal structure has been solved to 2.3 resolution. The x-ray crystal structure is useful for solving the structure of...
Progress in the synthesis, purification and crystallization of RNA has resulted in the determination of several X-ray crystal structures of RNA molecules over the past few years. Methods proven and under development will lead to future structure determinations and shed light on the structural basis ...
发明人: BENSON TIMOTHY E.,PRINCE DONALD BRYAN 摘要: The x-ray crystal structure of FemA and/or FemA-like proteins is useful for solving the structure of other molecules or molecular complexes, and identifying and/or designing modifiers of FemA activity.收藏...
Structure determination and refinement Structure determination by MAD has been described (Ferré-D’ Amaré et al., 1998a). Unlike crystal form II, crystal form III grows more readily in the presence of 0.1-0.3 mM cobalt (III) hexammine. Crystals of the unrelated P4-P6 domain RNA also grew...
Crystallization and structure determination of a symmetrical `football' complex of the mammalian mitochondrial Hsp60–Hsp10 chaperonins Nisemblat S; Parnas A; Yaniv O;.Crystallization and structure de- termination of a symmetrical 'football' complex of the mammalian mitochondrial Hsp60-... S Nisem...
The crystal structure of Na+,K+-ATPase has previously been elucidated using the enzyme from native sources such as porcine kidney and shark rectal gland. Here, the isolation, crystallization and first structure determination of bovine kidney Na+,K+-ATPase in a high-affinity E2–BeF3−–...
T. Version 1.2 of the Crystallography and NMR system. Nature protocols. 2 (11), 2728-2733 (2007). Brunger, A. T., et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta crystallographica. Section D, Biological. 54 (Pt 5), 905-921 (...
The recent crystal structure determination of T7 DNA polymerase complexed to a deoxynucleoside triphosphate and primer—template DNA has provided the first... Sylvie,Doublié,and,... - 《Current Opinion in Structural Biology》 被引量: 243发表: 1998年 High-throughput crystallization and structure de...
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA. Here we report the crystallization and structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) ... HC Losey,AJ Ruthenburg,GL Verdine - 《Nature S...
A fast strategy for Selenium derivatization and phasing of RNA structures Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein...