To discover the genes associated with sex inC. parvum, we sought to isolate specific parasite stages. We developed flow cytometry protocols to sort infected cells on the basis of the expression of fluorescent proteins by the parasite (Supplementary Fig.5). Figure3ashows sorts from cell culture an...
Today, Cryptosporidium parvum is well recognized as the cause of the disease cryptosporidiosis in humans and cattle and is one of the more opportunistic agents seen in patients with AIDS that occurs primarily in individuals with compromised immune systems. Currently, there is no totally effective ...
parvum in almost all life stages of the parasites, except the male gametes. Fig. 3 Expression of NFDQ1 in C. parvum life cycle stages indicated by immunofluorescence. a Immunofluorescence analysis of NFDQ1-HA in sporozoites. NFDQ1 sporozoites were fixed and stained with rabbit anti-HA mAb (...
Cryptosporidium parvumlife cycle.aThe excystation of a single oocyst releases four infective sporozoites. Using gliding motility as a means of locomotion, the sporozoites ultimately reach the microvilli of the intestinal epithelial cells.bThe parasite remains in the microvillar region inside a parasitoph...
Studies of in vitro excystation of Cryptosporidium parvum from calves showed that sporozoite yields were optimum when oocysts were treated with sodium hypo... DB Woodmansee - 《J Protozool》 被引量: 105发表: 1987年 Cryptosporidium: new developments in cell culture. Life cycleExcystationCell cult...
Cryopreservation of infectious Cryptosporidium parvum oocysts achieved through vitrification using high aspect ratio specimen containers Article Open access 16 July 2020 Transcriptional control of the Cryptosporidium life cycle Article 29 May 2024 Molecular identification and antiprotozoal activity of silver...
The chapter provides updated information on Cryptosporidium in the basic parasitology (e.g., taxonomy, life cycle, genotypes, and treatments), genomics (e.g., general features of the genomes, comparison between C. parvum and C. hominis genomes, genome maps, genome databases, and resources), ...
The C. parvum DNA replication machinery includes two unique replication protein A (RPA) heterotrimeric complexes that are expressed differentially during the parasite life cycle and in response to DNA damage (Rider and Zhu, 2008a). The RPA1 subunits provide the distinguishing characteristics of the ...
Today, Cryptosporidium parvum is well recognized as the cause of the disease cryptosporidiosis in humans and cattle and is one of the more opportunistic agents seen in patients with AIDS that occurs primarily in individuals with compromised immune systems. Currently, there is no totally effective ...
E., 2002, Comparison of fluorescence, antigen and PCR assays to detect Cryptosporidium parvum in fecal specimens, Diagn. Microbiol. Infect. Dis. 43:283–288. CAS Google Scholar Bier, J. W., 1991, Isolation of parasites on fruits and vegetables, Southeast Asian J. Trop. Med. Public ...