因为单颗粒冷冻电镜厚积薄发的技术突破，结构生物学在2013年底经历了一场“分辨率革命 (resolution revolution) ”，从此几家欢喜几家愁。欢喜的是，之前许多让科学家们束手无策的生物大分子们纷纷被揭开了神秘面纱；愁的是，作为研究者，为伊消得人憔悴、蓦然回首却在灯火阑珊处的乐趣少了许多。但是科研永无止境。
cryo-EMdrug discoverymembrane proteinsmultiprotein complexesSingle-particle cryogenic electron microscopy (cryo-EM) has been elevated to the mainstream of structural biology propelled by technological advancements in numerous fronts, including imaging analysis and the development of direct electron detectors. ...
With a well-behaved sample, cryo-EM can solve molecular structures with a resolution below 1.5 Å; a resolution level that was inconceivable only a few years ago. This rapid improvement of cryo-EM resolution has been called the “resolution revolution” and is the direct result of direct ...
Cryo-EM enabled the structure determination of TRP channel proteins from all seven subfamilies. The first major leap in the field came in 2013, when the structure of a deletion mutant of rat TRPV1 prepared in nanodiscs was solved to a resolution of 3.4 Å.21 TRPV1 has a strong link ...
2023年5月10日,清华大学生命学院李赛团队在Annual Review of Biophysics发表题为《冷冻电子断层成像的分辨率革命及伴随涌现的原位病毒学发现》(Cryo-Electron Tomography: The Resolution Revolution and a Surge of In Situ Virological Discoveries)的综述文章。该文章系统总结了cryo-ET领域近年来的技术革新、分辨率突破及...
因为单颗粒冷冻电镜厚积薄发的技术突破,结构生物学在2013年底经历了一场“分辨率革命 (resolution revolution) ”,从此几家欢喜几家愁。欢喜的是,之前许多让科学家们束手无策的生物大分子们纷纷被揭开了神秘面纱;愁的是,作为研究者,为伊消得人憔悴、蓦然回首却在灯火...
因此,科学家们首先需要将细胞或其他生物样本迅速冷冻在极低温下,固定它们在那一刻的状态,然后才能进行冷冻电镜下的观察。这也解释了冷冻电子显微镜成像(cryo-electron microscopy,简称为cryo-EM)中“冷冻”(cryogenic,简写为cryo-)一词的含义。 除了需要冷冻外,生物样品在冷冻电镜领域的应用还受到另一个重要限制,即样...
The Resolution Revolution and a Surge of In Situ Virological Discoveries[J].Annual review of biophysics, 2022.DOI:10.1146/annurev-biophys-092022-100958. 图1:Cryo-ET填补传统显微及结构生物学技术的空白地带(Sai Li; Trends in Biochemical Sciences, 2022; 47(2): 173-186) ...
2023年5月10日,清华大学生命学院李赛团队在Annual Review of Biophysics发表题为《冷冻电子断层成像的分辨率革命及伴随涌现的原位病毒学发现》(Cryo-Electron Tomography: The Resolution Revolution and a Surge of In Situ Virological Discoveries)的综述文章。该文章系统总结了cryo-ET领域近年来的技术革新、分辨率突破及...
因为单颗粒冷冻电镜厚积薄发的技术突破,结构生物学在2013年底经历了一场“分辨率革命 (resolution revolution) ”,从此几家欢喜几家愁。欢喜的是,之前许多让科学家们束手无策的生物大分子们纷纷被揭开了神秘面纱;愁的是,作为研究者,为伊消得人憔悴、蓦然回首却在灯火阑珊处的乐趣少了许多。