Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (G
CRY2 comprises a PHR domain and a non-conserved CCE domain. CIB1 contains an N-terminal domain (CIB1N) and a C-terminal domain (CIB1C). CIB1N is the CRY2-binding domain, whereas CIB1C, which contains the bHLH motif, interacts with DNA. (B) SEC (size exclusion chromatography) ...
腆着个老脸出来卖弄,弄巧成拙了吧[笑cry]//@两袖清风陈阿扁:大型翻车现场……//@paingod: http://t.cn/AiT6TQuq //@paingod: http://t.cn/AiT6Xo4L //@paingod: http://t.cn/AiT6qMkb //@闾丘露薇:請舉例,這樣才...
CRY2, CIB1, and CO form a protein complex in response to blue light to activate FT transcription, and the complex is regulated by the photoperiod and peaks at dusk along with higher FT expression. We also determined that CRY2 was recruited to the FT chromatin by CIB1 and CO and that ...