Reason :Gene cry Iac & cry II Ab control the cotton bollworms while cry Iab controls corn borer AIf both Assertion & Reason are True & the Reason is a correct explanation of the Assertion BIt both Assertion & Reason are True but Reason is not a correct explanation of the Assetion CIf As...
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Test results were validated with both positive and negative controls. Immunoblot of Cry1Ab. The experimental design was as described in section 'Insect bioassay with different tissues of Bt corn'. The collection of samples was as described in section 'ELISA of Cry1Ab'. The concentration of...
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To examine the responses of the secondary lepidopteran pest Spodoptera litura to two Bacillus thuringiensis (Bt) corn hybrids [5422Bt1 (Event Bt11), 5422CBCL (MON810)] expressing Cry1Ab, larval bioassays with Cry1Ab toxin, corn leaves or kernels and bagging on corn plants were conducted. The...
用TRIzol法提取各种处理条件下的玉米B73幼苗的总RNA, 用DNase I酶处理并以此为模板, 利用RevertAid First Strand cDNA Synthesis Kit并以Oligo-dT18引物将其反转录成单链cDNA, 保存于-80℃冰箱备用。 1.4ZmCRY1b和ZmCRY2基因的克隆 于NCBI 网站(http://www.ncbi.nlm.nih.gov/)查找ZmCRY1b(ZM02G13620)和...
Dhillon MK, Sharma HC (2009) Effects of Bacillus thuringi- ensis d-endotoxins Cry1Ab and Cry1Ac on the coccinellid beetle, Cheilomenes sexmaculatus (Coleoptera, Cocci- nellidae) under direct and indirect exposure conditions. Biocontr Sci Technol 19:407-420...
The cleavage of this helix exposes internal regions of domain I (probably hydrophobic regions of the protein), inducing oligomerization of the protein [59]. We compared oligomerization of the Cry1Ab trypsin-activated toxin with that of the protoxin after interaction with the cadherin fragment from...
cDNA-less controls for each primer pair were included in each run. Expression was calculated as 2-ΔΔCt and normalized to that of the reference gene. Function analysis of differentially expressed unigenes Go functional analysis provides GO functional classification annotation for DEUs as well as ...
Reason :Gene cry Iac & cry II Ab control the cotton bollworms while cry Iab controls corn borer View Solution Read the following and answer questions from Q.1. to Q.5. given below: Plants having foreign genes in their genome through genetic engineering are called transgenic plants. Genes ...