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DNA extraction, amplification and sequencing All materials were wiped with 75% ethanol before genomic DNA was isolated. Approximately 25–60 mg of root, rhizome, stem, cortex, fruit and seed material and 10–20 mg of leaf and flower material were prepared for DNA isolation. The materials ...
Total genomic DNA from fungal isolates was extracted using the MoBio PowerSoil DNA extraction kit according to the manufacturer’s instructions. DNA extracts were diluted 1:4 and this served as the working DNA concentration for PCR amplification. PCR reactions were carried out on Thermal Cycler 2720...
The blood concentrations of total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) decreased, and high-density lipoprotein (HDL) increased both in the crude polysaccharide- and rhamnose-enriched polysaccharide-treated rats. Superoxide ...
In addition, biosurfactants that diffuse poorly in agar may not be able to lyse blood cells [70, 71]. The reference M. antarctica strain was initially isolated from the bottom of a lake in Antarctica. Morita, et al. [43] proposed that MELs functionality may contribute to low-temperature ...
The LAMP assay was optimized to be compatible with purified DNA obtained upon an extraction step or DNA present in a crude plant tissue, both requiring sample pretreatement. For advanced field-based detection, a portable, lightweight, affordable and simple to operate device manufactured in the ...
To validate the CRISPR editing by Sanger sequencing, the PCR products were gel-purified using a gel extraction kit (QIAGEN) and cloned into the pGEM-T Easy vector (Promega) by TA cloning. Plasmid DNAs were submitted for Sanger sequencing to determine the DNA sequences in the edited mice. ...
It was reported that the protein-bound polysaccharides (PSPs) were nontoxic during prolonged use in the treatment of cancers by suppressing DNA/RNA synthesis and enhancing immune function [32,39,40]. In recent years, T. versicolor has attracted the attention of scientists not only as a source...
An ELISA kit was employed to ascertain if the extracts induce DNA fragmentation in cells at the specified IC50 concentrations. The BrdU provided in the kit is a non-radioactive thymidine analog that associates with genomic DNA to assess if the DNA has undergone fragmentation. In order to determi...
The extraction steps were performed using the ultrasonic technique, which is recognized as an innovative, effective, and environmentally friendly method [23]. Preliminary determinations of the chemical composition of the AmPS were conducted using spectrophotometric techniques. This revealed that the AmPS ...