这些cRNA探针可以有多种用途: 基因表达分析:cRNA探针常用于微阵列(Microarray)或RNA测序(RNA Sequencing)等技术中。这些探针通过与待检测样本中的RNA相互结合,可用于分析和比较不同条件下的基因表达水平。 原位杂交:制备的cRNA探针可以与组织样本中的特定RNA序列相互结合,用于原位杂交实验,从而在组织或细胞水平上可视化...
Formation of the T1-mRNA by trans-splicing was confirmed by RT-PCR analysis and DNA sequencing. Efficient trans-splicing required that competitive small t-antigen cis-splicing be inhibited by deletion of the small t-antigen 5' splice site. The T1-mRNA was not generated when the cryptic 5' ...
加州大学另一分校也在著名期刊《美国科学院通讯》(PNAS)上发文,揭示用“一管血”检测胞外RNA的新型液体活检方法。 2019年,加州大学圣地亚哥分校钟声教授领导的研究团队(联合H. Irene Su教授、钱煦教授)突破性地开发了一种名为“SILVER-seq”(Small Input Liquid Volume Extracellular RNA Sequencing)的低上样量exRNA...
transcriptional cascade in which MdWRKY1 is activated by light to increase the transcription of MdLNC499, which in turn induces MdERF109. The MdERF109 protein induces the expression of anthocyanin-related genes and the accumulation of anthocyanins in the early stages of apple coloration. Our resul...
基因表达分析:cRNA探针常用于微阵列(Microarray)或RNA测序(RNA Sequencing)等技术中。这些探针通过与待检测样本中的RNA相互结合,可用于分析和比较不同条件下的基因表达水平。 原位杂交:制备的cRNA探针可以与组织样本中的特定RNA序列相互结合,用于原位杂交实验,从而在组织或细胞水平上可视化目标RNA的空间分布和表达模式。
A nonisotopic heteroduplex complexity assay (HCA) was first validated by comparison with results of quasispecies complexity determined by subcloning and sequencing of amplicon products from the E2/NS1 hypervariable coding region (HVR). Subsequently, this methodology was applied to comparison of 20 ...
(2) constructing a probe plasmid for cRNA in-situ hybridization of the EAAT2; transforming the probe plasmid into bacteria, culturing the bacteria and sequencing; (3) preparing a probe for the cRNA in-situ hybridization of the EAAT2; and (4) detecting the in-situ hybridization of the cRNA...
(2) constructing a probe plasmid for cRNA in situ hybridization of the (i) ndrg2 gene, transforming bacteria and conducting amplification culture, and conducting Sequencing identification on the probe plasmid; (3) preparing an in situ hybridization probe of the (i) cRNA gene; and (4) ...