CRL4Cdt2: master coordinator of cell cycle progression and genome stability. Cell Cycle. 2011; 10 :241–249. doi: 10.4161/cc.10.2.14530. [ Cross Ref ]Abbas T, Dutta A. CRL4Cdt2: master coordinator of cell cycle progression and genome stability. Cell Cycle 2011 ; 10:241 - 9; http:...
Thus physical interaction of TDG with PCNA through the PIP degron is required for targeting TDG to the CRL4Cdt2 E3 ubiquitin ligase complex. Compared with forced expression of wild type TDG, CRL4Cdt2- resistant TDG (ΔPIP) slows cell proliferation and slightly increases the toxicity of 5-FU...
1 reagents referenced in Ubiquitin E3 ligase CRL4(CDT2/DCAF2) as a potential chemotherapeutic target for ovarian surface epithelial cancer.
PCNA provides a molecular platform for substrate recognition by the CRL4Cdt2 E3-ubiquitin ligase and p21 targeting during MVM infection required its interaction both with Cdt2 and PCNA. PCNA is also an important co-factor for MVM replication which can be antagonized by p21 in vitro. Expression ...
We also investigated for key CRL4 substrate adaptors required for ovarian cancer cells. Depleting Vprbp/Dcaf1 did not significantly affect ovarian cancer cell growth, even though it was expressed by ovarian cancer tissues. However, depleting Cdt2/Dcaf2 mimicked the pharmacological effects of MLN4924...
PIP degron proteins, substrates of CRL4(Cdt2), and not PIP boxes, interfere with DNA polymerase eta and kappa focus formation on UV damage PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase η and κ focus formation on UV damage. Nucleic Acids ...
PCNA loading does not trigger simultaneous destruction of all CRL4-Cdt2 substrates however; Cdt1 degradation begins nearly instantaneously with PCNA loading whereas p21 degradation is significantly delayed for example. As a consequence, the substrates of CRL4-Cdt2 are destroyed in a stereotypical ...
Antebi, M. Pagano, Regulation of the CRL4(Cdt2) ubiquitin ligase and cell-cycle exit by the SCF(Fbxo11) ubiquitin ligase, Mol. Cell 49 (2013) 1159-1166.Rossi M, Duan S, Jeong YT, et al. Regulation of the CRL4(Cdt2) ubiquitin ligase and cell-cycle exit by the SCF(Fbxo11) ...
CRL4(Cdt2) regulates cell proliferation and histone gene expression by targeting PR-Set7/Set8 for degradation. Mol Cell. 2010;40(1):9–21 [Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov’t]. CentralAbbas T, Shibata E, Park J, Jha S, Karnani N, Dutta A. ...
The cullin-based CRL4-CDT2 ubiquitin ligase is emerging as a master regulator of cell proliferation. CRL4-CDT2 prevents re-initiation of DNA replication during the same cell cycle “rereplication” through targeted degradation of CDT1, SET8 and p21 during S-phase of the cell cycle. We show...