In this study, we evaluated the effect of MVP on PRRSV infection in CRL2843CD163cell lines and porcine alveolar macrophages (PAMs). Our results showed that MVP expression was downregulated by PRRSV infection. Adenoviral overexpression of MVP inhibited PRRSV replication, whereas the siRNA knockdown ...
CRL2843CD163cells or PAMs were seeded onto 12-well plates or 24-well plates and infected with the Adv–MVP at a multiplicity of infection (MOI) of five for 24 h or 12 h, followed by infection with PRRSV (at an MOI of 1 or 0.1). CRL2843CD163cells or PAMs were harvested for ...