Download: Download full-size image Figure 1. PROTAC-induced target protein degradation (TPD). PROTAC binds either target protein or E3 enzyme (CRBN) to form a binary complex. Then a ternary complex (target protein – PROTAC – CRBN) forms, followed by ubiquitination, involving the CRL4A com...
Colorectal cancer (CRC) remains one of the most frequent malignancies with 177,000 new cases and 58,000 deaths per year in the USA1, with one-fifth of CRC patients presenting metastatic disease (mCRC). Currently, irinotecan in combination with fluorouracil is the first-line chemotherapy for mC...
In addition, the carboxylate form lead to bladder toxicity without showing efficacy when injected into humans [12]. Download : Download high-res image (288KB) Download : Download full-size image Fig. 1. Schematic diagram of CRLX101, a nanopharmceutical comprised of camptothecin conjugated to ...
Full size image This result suggests that TC-DPC repair represents the predominant DPC repair pathway in non-replicating or differentiated cells. To test the contribution of TC-DPC repair in non-replicating cells, we performed Alamar-Blue-based survival assays, which showed that non-replicating CSA...
Full-length AhR and PPARγ truncations were expressed in Escherichia coli. Our results showed that PPARγ binds AhR mainly on its DNA-binding domain (136–232aa). The N-terminal region of PPARγ (1–135aa) seemed to assist in binding AhR, whereas the C-terminal region of PPARγ, ...
The core domain-truncated TOP1 and C- terminus-truncated TOP1 failed to bind DCAF13, while the TOP1 core domain alone was able to bind DCAF13, albeit not as strongly as the full-length TOP1 (Fig. 7b). This is presumably due to its lost ability to form TOP1ccs. These results ...