The final PCR primer concentration was 500 nM in a volume of 10 μl with the SensiFast SYBR No-ROX kit (Bioline, London, UK). The following amplification program was used: an initial denaturation step at 95°C for 5 s followed by denaturation (10 s at 95°C), annealing (10 s...
Moreover, if DIS data with small hadronic invariant masses W 2 are not used in the analyses in order to reduce the sensi- tivity to higher twist contributions, the statistical potential of 123 Eur. Phys. J. C (2016) 76:471 0.14 HERA Charm 0.12 0.1 0.08 0.06 0.04 0.02 Data Q2 = 2.5...
Escherichia coli strain JM109 was used for the above cloning experiments and all clones were confirmed by sequencing. A. tumefaciens GV3101 was used for Agro-infiltration as previously described (Han et al. 2010). RNA library construction, small RNAs assembly, and downstream sequence analyses...
As a result, such "sensi- tivities" can be the key to virtual patients and personalised model-based care, as they provide an input–output relationship that reflects patient status and response to the treatment, as well as a metric that can then be used to titrate dose and thus care. ...
Jasmine virus H (JaVH) is a newly reported viral pathogen of jasmine in China and USA. To study the viral gene function and pathogenic mechanism, a full-length infectious clone of JaVH (pXT-JaVHFJ) was constructed under the control of the cauliflower mos
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