Processed data from the CRISPRi screens can also be interactively explored on CRISPRbrain (https://www.crisprbrain.org/screens/) by selecting ‘Astrocyte’ as the screening cell type. The public databases used in this study include Enrichr (https://maayanlab.cloud/Enrichr/), GENCODE (https:/...
中国科学院天津工业生物技术研究所王钰研究员与德国比勒费尔德大学(Bielefeld University)Volker F. Wendisch教授等在Engineering Microbiology上发表了标题为“Genome-scale CRISPRi screening: A powerful tool in engineering microbiology”的综述论文,...
Here we describe a robust protocol for CRISPR screening using human iPSCs. Because each screening is different and needs to be customized depending on the cell types and phenotypes of interest, we show an example of CRISPR knockdown screening using CRISPRi system to identify essential genes to ...
Fig. 1: CRISPRi screening of prostate cancer risk CREs. aSchematic of rCRE selection and sgRNA design.bDistribution of sgRNAs targeting rCREs or control regions. The barplot in the inset indicates the number of regions in the library.cThe cumulative distribution of depletion p-values of sgRNA ...
然而,目前缺乏重组蛋白生产相关功能基因的数据,阻碍了枯草芽胞杆菌细胞工厂的进一步发展。近日,来自江南大学的吴敬实验室在《Advanced Science》期刊上发表文章“Genome-Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis”,提出了一种结合全基因组CRISPRi筛选和靶向CRISPRa激活来...
1. Genga, R. M. J. et al. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell Reports 27, 708-718.e10 (2019). 2. Bester, A. C. et al. An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resista...
Hudson课题组发表在Nature Communications的“Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes”文章中,在集胞藻PCC 6803中开发了一个可诱导的CRISPRi基因阻遏文库以获得10498个克隆。 每个克隆表达dCas9和靶向蛋白编码开放阅读框或非编码RNA的sgRNA。 研究人员...
CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an L-proline exporter for L-proline hyperproduction 杂志:Nature Communications 发表时间:2022年2月 DOI:10.1038/s41467-022-28501-7. 通讯作者:天津工业生物所郑...
近日,清华大学化工系生物育种技术与装备团队和江南大学粮食发酵与食品生物制造国家工程研究中心白仲虎团队合作,在代谢工程领域国际知名期刊 Metabolic Engineering 上发表了题为“CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion”的文章,论文通讯作者...
Through large-scale CRISPRi screening, we discovered genetic modifiers in GBM cells, including ARPC4, PI4KA, ATP6V1A, UBA1, and NDUFV1, that regulated the efficacy of CAR T cell-mediated tumor killing. Furthermore, we discovered that TNFSF15 was upregulated in both ARPC4 and NDUFV1 ...