Read about IDT’s Alt-R CRISPR-Cas9 genome editing system and Cas9, the most common nuclease in CRISPR gene editing systems.
The ArciTect™ product family is a CRISPR-Cas9 genome editing system that uses ribonucleoprotein (RNP) complexes composed of purified Cas9 protein and custom synthetic guide RNA. Compared to previous technologies that utilize plasmid or mRNA-based systems, an RNP-based system enables efficient ...
[6]Lino CA, Harper JC, Carney JP, Timlin JA. Delivering CRISPR: a review of the challenges and approaches. Drug Deliv. 2018;25(1):1234-1257. doi:10.1080/10717544.2018.1474964 [7]Cheng H, Zhang F, Ding Y. CRISPR/Cas9 Delivery System Engineering for Genome Editing in Therapeutic Applic...
Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus are the best characterized CRISPR-associated nucleases. CRISPR/Cas9 genome editing system uses a RNA-guided endonuclease to introduce site-directed double-strand breaks (DSBs) in DNA
Are you genome editing with CRISPR-Cas9? Consider the Alt-R CRISPR-Cas9 kit—a customizable, end-to-end Cas9-CRISPR System offering best in class performance.
The discovery of CRISPR: Cas9 and its application as a powerful gene-editing tool has transformed the world of basic and applied science, especially the molecular biology dome. Also, the smooth, quick, flexible, and very efficient nature of this technology has enabled the biologists to alter ...
基因编辑技术 基因编辑技术 (genome editing) 可以精确地破坏、插入或替换基因组中特定位点的 DNA 序列, 在基因功能的研究和遗传疾病的治疗中发挥着巨大的作用。人工核酸内切酶 (engineered endonuclease, EEN) 介导的基因编辑技术极大地改善了早期基于同源重组的基因打靶技术 (gene targeting) 效率低的问题, 使得科研...
[8] He XY, Ren XH, Peng Y, et al. Aptamer/ peptide-functionalized genome-editing system for effective immune restoration through reversal of PD-L1-mediated cancer immunosuppression. Adv Mater, 2020, 32(17): 2000208. [9] Humbert O, Radtke S, Samuelson C, et al. Therapeutically relevant ...
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
For transfections into suspension cells, we recommend using theNeon Transfection System. Perform transfections using standard 24-well culture plates. This plate size is convenient when screening different gRNA...