elegans genomic sequences which can be effectively targeted by the CRISPR-Cas9 system, we created CRISPR-Tag as a DNA tagging tool in living human cells, enabling live-cell labeling of non-repetitive genes at the level of single cells and single loci. Although these tags are as small as ...
You can design your CRISPR gene knockout, knock-in, or tagging experiment, and order a complete set of reagents (including gRNAs) for it, with our free TrueDesign Genome Editor software. Design and order reagents for your entire CRISPR edit with the TrueDesign Genome Editor Contact us for ...
Fluorescent proteins have revolutionized biomedical research as they are easy to use for protein tagging, cope without fixation or permeabilization, and thus, enable live cell imaging in various models. Current methods allow easy and quick integration of fluorescent markers to endogenous genes of ...
(2017) CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol.doi: 10.1021/acschembio.7b00549 Xu MM, Pu Y, et al.. (2017) Dendritic cells but not macrophages sense tumor mitochondrial DNA for cross-priming through signal regulatory protein α signaling. ...
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Lu Y, Tian Y, Shen R, et al. Targeted, efficient sequence insertion and replacement in rice[J]. Nature Biotechnology, 2020, 38(12): 1402-1407.Lu Y, Ronald P C, Han B, et al. Rice protein tagging project: A call for international collaborations on genome-wide in-locus tagging of ...
近日，微生物技术国家重点实验室祁庆生团队侯进教授在Nucleic Acids Research发表题为“CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression inSaccharomyces cerevisiae”的论文，开发CRISPR转录调控新工具。侯进教授为论文通讯作者，研究团队博士生翟昊天为第一作者...
D. A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell 159, 635–646 (2014). Article CAS PubMed PubMed Central Google Scholar Fujita, T. & Fujii, H. Efficient isolation of specific genomic regions and identification of associated proteins by ...
[6] Ogrodzinski, Lydia, et al. "Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells." Iscience 26.7 (2023).