PredictIt is a real-money political prediction market, a stock market for politics. A project of Victoria University of Wellington, PredictIt has been established to research the way markets can forecast future events. F Fyre Media Fyre Media is the developer of Fyre, an on-demand service that...
FastTree approximately maximum-likelihood phylogenetic trees; Extended Data Figs.9aand10a–c) and the presence of a promoter sequence within 250 bp upstream of the Racr candidate (promoter prediction using Bprom and
FastTree approximately maximum-likelihood phylogenetic trees; Extended Data Figs.9aand10a–c) and the presence of a promoter sequence within 250 bp upstream of the Racr candidate (promoter prediction using Bprom and
Prediction of protein structures and pairwise comparison The structures of phage and bacterial proteins were predicted using AlphaFold2 (v. 2.1.2) (–model_preset = monomer)40. For each protein, 5 models were generated, and we selected the best model (ranked_0.pdb) determined by the average ...
A pair of 25 nt oligos containing the appropriate overhang was then ligated into the vector by mixing 1 μL of the cut vector (normalized to 100-200 ng/μL), 0.5 μL of each primer at a 100 μM stock concentration, 2 μL of 10x T4 DNA ligase buffer, and 1 μL of T4 DNA ...
(2 µL of a 50 ng/µL stock) in a 0.1 cm electroporation cuvette (Bio-Rad #1652089). The cuvette was placed in the electroporator (Bio-Rad #411BR) and pulsed at 1.8 kV for a duration of 5 ms. In total, 1.975 mL recovery media was added to each cuvette and...