分析模式 该R包可以进行两种模式的分析,一种是“quick”模式,一种是“step by step”模式。 Quick模式 01 加载R包 代码语言:javascript 复制 library(MAGeCKFlute)library(ggplot2) 02 加载数据 代码语言:javascript 复制 #MAGeCK分析结果数据-gene file1=file.path(system.file("extdata",package="MAGeCKFlute"),...
Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts Part of the book series: Methods in Molecular Biology (MIMB, volume 2162) 38k Accesses 2 Altmetric This is a preview of subscription content, log in via an institution to check ac...
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Dual substrate/sgRNA libraries for multiplex CRISPR screens were constructed by first generating a library of substrates fused to GFP in the context of the GPS lentiviral vector, followed by the addition of a downstream U6-sgRNA cassette encoding a library of CRISPR sgRNAs. To generate a substrate...
2015). Step by step, five different gene deletions and concomitant insertions of ganA under control of the gut promoter Pgut, were introduced in REG19 to obtain the strains JA-Bs34, JA-Bs35, JA-Bs36, JA-Bs37 and JA-Bs40 (see Table 1). For induction of ganA expression, the over...
5. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell. 2013 Sep 12;154(6):1370-9.6. Sánchez-Rivera FJ, Jacks T. Applications of the CRISPR-Cas9 system in ...
CRISPR :英文全称是 Clustered Regularly Interspaced Short Palindromic Repeats,规律成簇的间隔短回文重复,是大多数细菌及古细菌中一种不断进化适应的免疫防御机制。 CRISPR/Cas :英文全称是 Clustered regularly interspaced shortpalindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, 是原核生物的免疫系统,用来...
Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events in Vivo CRISPR-Cas9 based technology is currently the most flexible means to create targeted mutations by recombination or indel mutations by non-homologous end joining. During mouse transgenesis, recombinant and indel alle...
基因编辑是一种对靶基因或转录产物进行敲除、插入和定点突变等精确修饰的基因工程技术,主要通过人工核酸酶实现对基因组的特定基因序列的敲除、插入或精确修饰。目前,主要有三大基因编辑技术,包括:锌指核酸酶 (Zinc finger nucleases; ZFNs) 技术,转录激活因子样效应物核酸酶 (transcription activator-like (TAL) effector...
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPRCas-Mediated Genome Engineering. Cell, Volume 153,Issue 4,910-918. MoSnapShot: CRISPR-RNA-Guided Adaptive Immune Systems,Cell 163. 作者:霸霸 配图来源:网络 关于CRISPR,都有哪些核心文献呢?请回复CRISPR,霸霸已经下载好,统一给...