In this review article, we will provide an overview of the current state of the field of CRISPR-based gene editing in cancer therapy, highlighting the most promising results and the challenges that still need to be overcome. We will describe the different CRISPR-based strategies that have been ...
nature nature cell biology review articles article Review Article Published: 08 January 2021 CRISPR technologies for precise epigenome editingMuneaki Nakamura, Yuchen Gao, Antonia A. Dominguez & Lei S. Qi Nature Cell Biology volume 23, pages 11–22 (2021)Cite this article ...
Review Article Open access Published: 15 May 2018 The CRISPR tool kit for genome editing and beyond Mazhar Adli Nature Communications volume 9, Article number: 1911 (2018) Cite this article 245k Accesses 1181 Citations 106 Altmetric Metrics details Abstract CRISPR is becoming an indispensable ...
This review evaluates the benefits of chemically synthesized gRNAs as well as the integration of chemical amendments to improve gene editing efficiencies. CRISPR is an indispensable means in biological investigations and is now as well transforming varied fields of biotechnology and agriculture. Recent ...
2024年4月29日,Nature发表文章‘ChatGPTfor CRISPR’ creates new gene-editing tools,介绍了人工智能助力创建基因编辑新工具。 为了发现之前未知的CRISPR基因编辑系统,研究人员对温泉、泥炭沼泽、粪便甚至酸奶中的微生物进行不断的探索。现在,得益于生成人工智能的进步,他们也许只需按一下按钮,就能设计出这些系统。
在2014年,Doudna、Charpentier,Zhang均在顶级学术期刊发表了关于CRISPR-Cas的Review,前两人合作发表在Science杂志,后者与Patrick D. Hsu及Eric S. Lander合作一起发表在Cell杂志。我在细读他们的文章时发现:在2篇文章中都用了时间轴描述CRISPR-Cas的重大事件,Zhang在描述2013年第一次实现人类基因组编辑的时候,仅引用了...
This review provided a brief overview of the function and construction of CRISPR/Cas9 system in S. cerevisiae gene editing, introduced the design rules and representative design tools of sgRNA targeting sequences, as well as summarized the multiple-gene editing strategy. Moreover, the review ...
to accelerate genetic gains through targeted genetic modifications, producing crops that can overcome the shifting climate realities. This review focuses on how revolutionary genome editing tools can be directly implemented into breeding programs for cereal crop improvement to rapidly counteract many of the...
CRISP R–Cas9Structures a nd Mechanisms. Fuguo Jiang and Jennifer A. Doudna. Annual Review ofBiophysics 2017 46:1, 505-529 因HR对于DSB的修复效率较低,即对于CRIPSR-Cas9剪刀造成的DSB,细胞常以NHEJ的方式处理。然而NHEJ对于DNA双链的修复又会在剪切位点引入随机的插入或缺失,这对于基因治疗来说无疑是需...
During thereview process of Casgevy- a CRISPR cell therapy designed to treat sickle cell disease - the FDA focused on the potential for the medicine to cause off-target edits, highlighting thatpeople who carry rare genetic variants may be at higher risk. The FDAguidance on human genome editing...