2007 . CRISPR Recognition Tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats . BMC Bioinform. 8 : 209 .Bland C , Ramsey T , Sabree F , Lowe M , Brown K , Kyrpides N & Hugenholtz P ( 2007 ) CRISPR recognition tool (CRT) : a tool for...
CRISPRRecognition Tool(CRT)识别基因组中的CRISPR结构.通过确定cas蛋白的位置来确定CRISPRs的方向,全基因组比对并构建进化树.经过分析发现,这个物种主要有2套CRISPR... 周慧玥 - 中国科学院大学 被引量: 0发表: 2015年 Sensitive and reliable miRNA analysis based on cyclic reverse transcription and CRISPR-Cas12...
CRT - [software] - CRISPR Recognition Tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats. MetaCRAST - [software] - A tool detects CRISPR arrays in raw, unassembled metagenomes. metaCRT - [software] - A software for de novo prediction of CRISPR....
Firstly, despite the presence of hundreds of naturally found meganucleases, each of them has a unique recognition sequence. Thus, the probability of finding a meganuclease that targets a desired locus was still low. Secondly, and more critically, the majority of induced DSBs are repaired through ...
Charles Bland et al., CRISPR Recognition Tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats, BMC Bioinformatics 8, no. 1 (2007): 209. INSTALLATION You need to install these dependencies first: ...
Shah, SA. et al. Protospacer recognition motifs: mixed identities and functional diversity. RNA Biology, 10, 5 (2013), 891–899. doi: 10.4161/rna.23764. Sharma, P.K. et al. Tailoring microalgae for efficient biofuel production. Frontiers in Marine Science, 5 (2018), 382. doi: 10.3389/...
Primers were designed to create 89 bp long dsDNA inserts by PCR, containing the unique 20 nt spacer sequence, the hammerhead ribozyme, the 6 bp inverted repeat of the 5′-end of the spacer sequence and the BsaI type II restriction enzyme recognition sites. ...
of bacterial TnpB_IS605 are widespread in diverse eukaryotic transposable elements. Mob. DNA 4, 12 (2013).9. Saito, M., Xu, P., Faure, G. et al. Fanzor is a eukaryotic programmable RNA-guided endonuclease. Nature 620, 660–668 (2023).10. Karvelis, T. et al. PAM recognition by...
Although different types of CRISPR/Cas systems exhibit similarities in their genome editing patterns, the recognition and cleavage methods and their underlying machineries are different, which directly determine how to choose the optimal CRISPR/Cas tools for individual experimental purposes. To simplify and...
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