(2011) Genome engineering with zinc-finger nucleases. Genetics. 188(4): 773-82. 此外,如果锌指DNA结合域与目的DNA序列能够完美配对,即便只含3个ZF结构的ZFN也能在基因组中特异性地结合18bp长度的序列。通过研究者长期的努力,识别每一种三联碱基的64种锌指组合中已有大部分被发现并编撰成目录,这些相关数据也...
研究成果多次发表在Nature、PNAS、Genetics、G3和EMBO J等国际学术期刊。生物通:基因组暗物质虽然逐渐摆脱了“垃圾DNA”的名号,但依然是说不清,道不明,要阐明这些元件的作用,您的研究组采用了什么分析思路?所得到的结果是否与您预期的相符? 高博士:传统的研究认为,编码人类蛋白质的基因只有2~3万个,且只占全...
The article focuses on a research conducted by researcher Lei Qi and colleagues related to reverse transcription and clustered, regularly interspaced, short palindromic repeats (CRISPR) system. According to the research, Cas9 endonuclease allows targeted RNA silencing. It also mentions that CRISPR system...
3、Paix, Alexandre et al. “Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.” Genetics vol. 198,4 (2014): 1347-56. doi:10.1534/genetics.114.170423 4、Banan, Mehdi. “Recent advances in CRISPR/Cas9-mediated knock-ins in ma...
Nature genetics 47.5 (2015): 469-478. Novel CRISPR Applications Endogenous Luciferase Reporter Common protocol to measure the affect of extracellular signals on protein expression is to transfect a cell line with a plasmid expressing a reporter (such as luciferase) under the control of known ...
[2] ZHANG F, WEN Y, GUO X. CRISPR/Cas9 for genome editing: progress, implications and challenges[J/OL]. Human Molecular Genetics, 2014, 23(R1): R40-46. DOI:10.1093/hmg/ddu125. [3] 【呕心巨制&史上最全】CRISPR专利之争的前世今生和未来[EB/OL]//知乎专栏. [2023-06-12].https://zhu...
2015年，《科学》将它评为年度突破；助力这项技术诞生的科学家们也先后获得了有“科学界奥斯卡”之称的“突破奖”（Breakthrough Prize），在分子生物学界影响深远的“格鲁伯遗传学奖”（Gruber Genetics Prize），以及表彰重大生物医学突破的“沃伦·阿尔珀特奖”（Warren Alpert Prize）。在2014年12月的《环球科学》...
1. Carroll D. (2011). Genome engineering with zinc-finger nucleases. Genetics, 188(4), 773–782. https://doi.org/10.1534/genetics.111.131433 2. Joung, J. K., & Sander, J. D. (2013). TALENs: a widely applicable technology for targeted genome editing. Nature reviews. Molecular cell bi...
Check University Websites:Universities often host guest lectures and seminars by leading scientists in genetics and biotechnology. These are sometimes streamed online for free. Biotech Companies:Companies like CRISPR Therapeutics, Editas Medicine, and others occasionally offer webinars and workshops that ca...
DNA双链的糖-磷酸骨架为橙色,碱基显示为蓝色,在ZF结合位点两侧的DNA区域间距为6bp。该示意图由Smith等人在2000年根据锌指蛋白与DNA结合的晶体结构数据所编译而来。图片来源:Carroll D. (2011) Genome engineering with zinc-finger nucleases. Genetics. 188(4): 773-82....