CRISPR/CAS DROPOUT SCREENING PLATFORM TO REVEAL GENETIC VULNERABILITIES ASSOCIATED WITH TAU AGGREGATIONCas-protein-ready tau bio sensor cells, CRISPR/Cas synergistic activation mediator (SAM)-ready tau biosensor cells, and methods of making and using such cells to screen for genetic vulnerability ...
Also known as dropout screens. Cells with the phenotype of interest are depleted in the screen; other cells are maintained. Effective dose The intensity of a perturbation (such as a drug or virus) that causes an effect (such as cell death) in a specified percentage of cells. Screening hits...
Combining the endonuclease Cas9 and a pooled guide RNA library allows for systematic screenings of genes associated with a growth disadvantage or lethal phenotype under various conditions in organisms and tissues. Here, we describe a complete protocol for scalable CRISPR/Cas9-based dropout screening ...
et al. A CRISPR dropout screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia. Cell Rep. 17, 1193–1205 (2016). Article CAS PubMed PubMed Central Google Scholar Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators...
Moreover, an arrayed activation screen of 1,634 human transcription factors uncovered 11 novel regulators of the cellular prion protein PrPC, screening with a pooled version of the ablation library led to the identification of 5 novel modifiers of autophagy that otherwise went undetected, and ‘...
Next, we sought to understand how gRNA position within the CDS of the target gene impacts gRNA activity. Given that most gRNAs in Project Achilles were located in the first 50% of the target CDS by design, we obtained a different screening dataset; we downloaded data from a genome-wide fit...
(Additional file 1: Fig. 2A), and repeated DrugZ analysis on published CRISPR data using the Brunello library [64] (Additional file 1: Fig. 2C). We found that the results were largely unchanged when moving between GRCh37 and GRCh38. Results of the analysis after Exorcise with the T2T ...
Pooled CRISPR screening data in cancer cell lines is limited to Log2(fold changes) for PMAIP1 sgRNAs and is available upon request. RNA-seq data processing and pooled CRISPR NGS were conducted using open source software as indicated in the key resources table. Acknowledgments We would like ...
Teichmann and colleagues could use their dropout screen in the Th0 arm to assess genes that are essential for “activation,” and then compare between Th0 and Th2 arms to assess genes that are essential for “differentiation.” By combining these data with RNA-seq for gene expression, ATAC-...
1c). We investigated if low amino acid conditions altered transporter gene expression; low-Arg and low-Leu screening conditions increased expression of most genes in our SLC7 transporter panel, but not all of them, consistent with results for HEK293T cells in single amino acid dropout media35...