In order to construct the CRISPR-Cas9 complex, it is necessary to design and synthesize the Cas9 nucleic acid sequence. 2.接着需要选择适当的crispr RNA或gRNA序列,与cas9相结合以指导靶向基因组的位置。 Then, an appropriate CRISPR RNA or gRNA sequence needs to be selected to guide the Cas9 to ...
and the expressions of the fibrogenic genes Fibronectin and Collagen I were promoted.【Conclusion】 CRISPR/Cas9 technique was used to construct the renal tubular epithelial cell line of PMEPA1 knockout TCMK1 mice successfully, which established the cell model for the functional study of PMEPA1 pro...
osiaa11-1-2和osiaa11-17在第1个靶点缺失24个碱基,在第2个靶点处为单碱基缺失,但2个突变体植株缺失的碱基类别有差异,易引起表达产物中氨基酸残基丢失和移码翻译;osiaa11-22-1突变体则在2个靶点都缺失24个碱基,共形成外显子区域丢失48个碱基,会造成翻译产物缺失16个氨基酸;osiaa11-11-1和osiaa11-19-2突...
The clustered regularly interspersed palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9) system is a powerful tool for genome editing that can be used to construct a guide RNA (gRNA) library for genetic screening. For gRNA design, one must know the sequence of the 20-mer flanking ...