CRISPR-Cas9 for cancer therapy: opportunities and challenges. Cancer Lett. 2019. https://doi.org/10.1016/j.canlet.2019.01.017. Article PubMed PubMed Central Google Scholar Wang K, Jin Q, Ruan D, Yang Y, Liu Q, Wu H, et al. Cre-dependent Cas9-expressing pigs enable efficient in ...
R.R. is supported by the European Research Council (Consolidator Grants PACA-MET (819642) and MSCA-ITN-ETN (861196)), the Deutsche Forschungsgemeinschaft (DFG RA1629/2-1; SFB1321), the German Cancer Consortium and the Deutsche Krebshilfe (70114314). Author information Author notes These ...
41 We did this to overcome the lack of endogenous TTAA at suitable proximity to the Cas9 cleavage site. In adopting this approach, we considered the potential impact on expression of ADRB2 but reasoned the main two parameters, codon usage43 and location in the gene,44 were relatively risk ...
CHSY1 promotes CD8+ T cell exhaustion through activation of succinate metabolism pathway leading to colorectal cancer liver metastasis based on CRISPR/Cas9 screening Guangshun Sun, Siqi Zhao, Zhongguo Fan, Yuliang Wang, Hanyuan Liu, Hengsong Cao, Guoqiang Sun, Tian Huang, Hongzhou Cai,...
Genome-wide CRISPR-Cas9 screens in a setting of positive selection have discovered gene mu- tations that confer drug resistance, resistance to bacterial toxins and genes involved in metastasis [1, 4, 5, 7, 10]. For instance, using this approach Chen et al. discovered key genes involved in ...
J. et al. Genomic copy number dictates a gene-independent cell response to CRISPR/Cas9 targeting. Cancer Discov. 6, 914–929 (2016). Article CAS PubMed PubMed Central Google Scholar Munoz, D. M. et al. CRISPR screens provide a comprehensive assessment of cancer vulnerabilities but generate...
After 2 h of incubation the cells are again washed with Krebs Ringer HEPES (KRH)buffer without glucose and incubated at 37ᵒC.Repeated washing of cells with ice cold KRH buffer. Later the cells are incubated for 10 min at room temperature and transferred to a tube and vortexed gently...
Genetically fusing protein domains to Cas9 has yielded several transformative technologies; however, the genetic modifications are limited to natural polypeptide chains at the Cas9 termini, which excludes a diverse array of molecules useful for gene edit
(BEs) exploit chemical conversion of DNA bases to introduce permanent sequence changes without DSB. Shown is the conversion of C to U, the correction of the complementary base from G to A triggered by the Cas9 nickase activity, and the eventual establishment of a T-A base pair after DNA ...
25. We and others have shown that disrupting the NKG2A-HLA-E axis using NKG2A-blocking antibodies, such as monalizumab or CRISPR/Cas9-based disruption ofKLRC1in NK cells, can significantly enhance NK cell cytotoxicity against multiple myeloma23,24,25. We further observed that various AML cell...