CRISPR/Cas9基因编辑系统主要由两部分组成:相当于“扳手”作用的Cas9蛋白与相当于“螺纹钉”作用的CRISPR向导RNA(guide RNA,gRNA)。“螺纹钉”负责对靶位点进行定位,并招募和激活Cas9蛋白;Cas9蛋白则负责切割DNA(图2)。 图2. "分子改造器" CRISPR/Cas9 实现该“分子改造器”的方法有质粒转染、体外转录RNA、病毒包...
The IDT guide RNA design tool has 3 modes (accessible using the tabs at the top), as shown in Figure 1 (below). Figure 1. Screen capture of guide RNA design tool options. There are 3 options: “Search for predesigned gRNA,”“Design custom gRNA,” and “CRISPR-Cas9 gRNA checker.” ...
Cas9 guide RNA design tool Select from our predesigned gRNAs targeting human, mouse, rat, zebrafish, or nematode genes. For other species, use our proprietary algorithms to design custom gRNAs. For protospacer designs of your own or from publications, use our design checker tool to assess targeti...
Generate CRISPR-Cas9 guide RNAs (gRNAs, such as crRNA and sgRNA) targeting any sequence from any species. Currently, analysis of off-target effects against human, mouse, rat, zebrafish, orC. elegansgenes are available. For HDR experiment designs, please see the followingHDR design tool. ...
Select from predesigned Alt-R CRISPR-Cas9 guide RNAs (gRNAs, such as crRNA and sgRNA) targeting human, mouse, rat, zebrafish, or C. elegans gene targets.
Potential off-target effects of the selected sgRNAs were analyzed using the CRISPR-Cas9 guide RNA design checker developed by IDT, available at https://www.idtdna.com/site/order/designtool/index/CRISPR_SEQUENCE (accessed on 23 January 2023); and genes with a higher score for potential off-...