3a). This effect cannot be explained by differences in RNA expression level, which are uncorrelated with RNA editing rates across both non-eCLIP and eCLIP genes (|R2 | values < 0.04 across all replicates) (Fig. 3b). If nickase Cas9-APOBEC fusion co-expressed with gRNA binds to ...
The characteristics of CRISPR/Cas9-induced gene mutations have been carefully described in a small number of plant species. Mutation frequencies have ranged from 2.7% to 100% and are largely dependent on the promoter used to drive the expression ofCas99. InArabidopsisandCamelina sativa, mutations m...
vaginalis with different clinical phenotypes could be explained by different cytotoxicity of the strains, presumably based on disparate gene content. The contribution of horizontal gene transfer to shaping the genomes of G. vaginalis is acknowledged. The CRISPR loci of the recently discovered CRISPR/Cas...
Specific recognition of these repeats by Cas1-Cas2 restricts integration of viral DNA to the CRISPR array, allowing it to be used for immunity and avoiding the potentially fatal effects of inserting viral DNA in the wrong place, explained UC Berkeley graduate student Addison Wright and one of t...
The CRISPR/Cas system is known to act as an adaptive and heritable immune system in Eubacteria and Archaea. Immunity is encoded in an array of spacer sequences. Each spacer can provide specific immunity to invasive elements that carry the same or a simil
The multiple alleles in these cases cannot simply be explained by parental inheritance. The summary statements in our Correspondence reflect observations of a secondary outcome following successful achievement of the primary outcome using CRISPR to treat blindness in Pde6b/rd1 mice. As the scientific ...
has found that a system called CRISPR-Cas10 is superior at targeting rapidly mutating viruses. While CRISPR-Cas9 requires specific DNA sequences on the virus to be intact in order to cut it, the researchers found that CRISPR-Cas10, in the bacteria Staphylococcus epidermidis, can mount an effec...
This could be explained at least by the following three possibilities. (1) Several transfected founder cells happened to start together with a different subset of mutations induced by multiplexed CRISPR/Cas9 genome editing and developed into a single tumor nodule. (2) Some mutations were first ...
the allelic imbalance could be explained by a dinucleotide repeat insertion preventing adequate amplification of the affected allele by long-range PCR. In other cases, the homozygosity may be caused by larger events induced by CRISPR-Cas9 that result in failed amplification of the affected allele, ...
13b). This can be explained by the binding position of the visible 13-bp DNA duplex in the Csy-AcrIF24-dsDNASP complex, which shows the fluorescein label at the 3′ end of the target strand will cause negligible sterical hindrance (Fig. 6d). Therefore, we used the results obtained ...