Crhr1-IRES-Cre 品系名:C57BL/6Smoc-Crhr1em1(IRES-Cre)Smoc 品系状态:精子冻存 | 目录号:NM-KI-233206 你也可能感兴趣 Tamoxifen诱导Cre-ERT2小鼠 使用指南 Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2...
使用敲入小鼠(3-8 个月大的雌雄均可)将基因表达限制在 Barr CRH神经元中,该小鼠的 CRH 基因座下游具有内部核糖体进入位点 (ires) 连接的 Cre 重组酶基因(CRH Cre小鼠,Jax 实验室 #012704)。 4.2 量化和统计分析 所有数据均以平均值±SEM 表示(除非另有说明)。样本量是根据经验估计的,与其他已发表的研究相...
(1)在CRH-IRES-Cre小鼠的PVN脑区注射同时含有rAAV-CRH-CRE-WPRE-hGHpolyA和化学遗传学激活病毒rAAV-hM3D(Gq)-mCherry的140-160nl液体,其中rAAV-CRH-CRE-WPRE-hGHpolyA和化学遗传学激活病毒rAAV-hM3D(Gq)-mCherry的病毒滴度都为2.4-2.6×10 12
Optogenetic activation of Crh-IRES-Cre PVN neurons by cre-dependent viral expression of channelrhodopsin.Jaclyn, I. Wamsteeker CusulinTamás, FüzesiAlan, G. WattsJaideep, S. Bains
S. Characterization of corticotropin-releasing hormone neurons in the paraventricular nucleus of the hypothalamus of Crh-IRES-cre mutant mice. PLoS ONE 8, e64943 (2013). Article CAS Google Scholar Lerner, T. N. et al. Intact-brain analyses reveal distinct information carried by SNc dopamine ...
品系名:C57BL/6Smoc-Crhr1em1(IRES-Cre)Smoc 品系状态:精子冻存 | 目录号:NM-KI-233206 Crhr2-Flox 品系名:C57BL/6Smoc-Crhr2em1(flox)Smoc 品系状态:在研 | 目录号:NM-CKO-242072 你也可能感兴趣 Tamoxifen诱导Cre-ERT2小鼠 使用指南 Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当...
For experiments, Crh-IRES-Cre+/+ mice and offspring derived from crosses of homozygous Crh-IRES-Cre and Ai14 genotypes were used. Until the first procedure mice were group-housed, then single-housed on a 12:12 h light/dark schedule (lights on at 07:00 hours) with ad libitum access ...
最近,人们使用 CRH CRE小鼠探索了这些 Barrington 核 (Barr CRH ) 中的 CRH 阳性神经元的作用,以便对其活动进行特定的光和化学遗传学操控。这些研究表明,Barr CRH神经元具有谷氨酸能,它们的激活会引起膀胱收缩,而增加它们的兴奋性会增加排尿的可能性。Barrington 核中雌激素受体 1 型阳性神经元的第二个较小的亚群...
Fast glutamate and GABA transmission in Crh-IRES-Cre;Ai14 tdTomato neurons.Jaclyn I. Wamsteeker CusulinTamás FüzesiAlan G. WattsJaideep S. Bains
Crh-IRES-Cre;Ai14 mice were treated with either vehicle or metyrapone and subsequently exposed to a single white noise stress as described above (Figs. 2 and 3). We then prepared acute brain slices containing the PVN 60 min after white noise stress and analyzed parameters of intrinsic ...