Genetically hypertensive inbred rat strains provide the advantages of reduced genetic and pathogenic complexity, as hypertensive SHR lines share an overlapping set of hypertension alleles [17], and environmental variation that is limited by shared housing and breeding conditions. Previous studies of SHR-A...
To genetically validate that mutation ofARFTF17was responsible for the mutant phenotype, we obtained a different EMS-generated B73 allele from the MEMD database (http://elabcaas.cn/memd/public/index.html#/)15, and designated itfka1-2. Thefka1-2allele also contains a C to T transition in...
A:The ability to monitor oxygen consumption in live cells has provided an immense benefit when used in isolation to evaluate metabolic changes in cells in different experimental conditions (i.e., while cells are proliferating, during and after cellular differentiation, in response to genetic or phar...
To determine if therapy-induced senescence can alter the PDAC TME, we evaluated the response to T/P treatment in two mouse models of PDAC that recapitulate the histopathology and stromal responses observed clinically: the Pdx1-Cre;LSL-KRASG12D;Trp53fl/wt (KPCflox) genetically engineered mouse ...
We injected the AAV-DIO-ChR2:YFP in the PBN to optogenetically stimulate the CGRP neurons, and we also injected the AAV-DIO-mCherry in the CeA of the same mice to label the CGRPR cells in the CeAl. Dense, perisomatic green fibers were observed surrounding mCherry-positive cell bodies ...
Infectious disease is recognized as the greatest threat to the endangered chimpanzees made famous by the groundbreaking work of Dr. Jane Goodall at Gombe National Park (GNP), Tanzania. The permeable boundary of this small protected area allows for regula
analyze and measure the health of cells in the human body, and react accordingly. Then, custom-coded bacteria could be used to deliver medication in specific doses required by different body parts at different times of day, based specifically on the condition of the target cells in real time...
In contrast, there was no difference between the proportions of cells at different stages of the cell cycle between CDCA7L knockdown and control cell lines (Supplementary Fig. 7). Following on from this we examined the impact of CDCA7L expression on MM patient outcome in five independent ...
Different stromal cells were analysed in sections of LN from bone marrow (BM) chimeras where ILC3s were heavily depleted. No gross effects were evident in LN architecture with normal segregation of B and T cell areas. Lymphatic vessels and high endothelial venules appeared comparable between ...
We demonstrate both by binding studies on genetically engineered soluble receptors and by the mitogenic response of growth factor-dependent cell lines that this splice variant of FGFR3 (FGFR3 IIIb), by binding only acidic FGF (aFGF/FGF-1), has the most restricted ligand binding properties of ...