cre诱导形成DTR转基因小鼠(iDTR),其中cre介导的STOP卡带切除使细胞对白喉毒素(DT)敏感。将iDTR品系分别与T细胞和B细胞特异性的CD4-Cre和CD19-Cre品系杂交,观察到DT暴露后T细胞和B细胞的有效消融。在少突胶质细胞中表达Cre重组酶的MOGi-Cre/iDTR双转基因小鼠中,我们观察到腹腔注射DT后髓鞘丢失。因此,DT可以穿过血脑...
cre诱导形成DTR转基因小鼠(iDTR),其中cre介导的STOP卡带切除使细胞对白喉毒素(DT)敏感。将iDTR品系分别与T细胞和B细胞特异性的CD4-Cre和CD19-Cre品系杂交,观察到DT暴露后T细胞和B细胞的有效消融。在少突胶质细胞中表达Cre重组酶的MOGi-Cre/iDTR双转基因小鼠中,我们观察到腹腔注射DT后髓鞘丢失。因此,DT可以穿过血脑...
Oligodendrocyte depletion with Diphtheria Toxin in MOGi-Cre/iDTR mice.Aniket, GhoshNatalia, ManriqueHoyosAaron, VoigtJörg, B. SchulzMario, KreutzfeldtDoron, MerklerMikael, Simons
Methods : We utilize of a model of hypoxia-induced retinopathy to model the ischemia and neovascularization of DR. The GFAP-CreERT2:Rosa26iDTR mouse line renders astrocytes sensitive to diphtheria toxin (DTx) following tamoxifen administration and induces astrocyte hypertrophy. To test for astrocyte ...
Immunofluorescence and quantitation of SIM1 neurons in PVN and SON of iDTR and Sim1Cre mice.Dong, XiJeff, RoizenMeizan, LaiNilay, GandhiBassil, Kublaoui
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...