Deverman BE, Pravdo PL, Simpson BP et al (2016) Cre-dependent se- lection yields AAV variants for widespread gene transfer to the adult brain. Nat Biotechnol 34:204-209Deverman, B. E. et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. ...
We used an adeno-associated virus (AAV) vector for human TDP-43 expression targeted to the substantia nigra (SN) of...doi:10.1096/fj.201701529RRGrames Mychal S.Dayton Robert D.Jackson Kasey L.Richard Adam D.Lu XiaohongKlein Ronald L....
Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of tropisms. To expand their utility, we evolved AAV capsids to efficiently transduce specific cell types in adult mouse brains. Building upon our...
252. Using Cre-Dependent In Vivo Selection to Identify AAV Variants That Enable Efficient and Widespread Gene Transfer to the Adult Central Nervous Systemdoi:10.1016/S1525-0016(16)33061-1Deverman, Benjamin E.Pravdo, Piers L.Simpson, Bryan P....
AAV vectors that efficiently transduce the mouse brain after intravenous injection are generated with a CRE-dependent selection system. Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer1,2,3,4,5,6. However,
Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, leading to effective recombination selectively in GFP-labeled cells. Further, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene ...