Organoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this study, we esta...
类器官 (Organoids) 技术:类器官是细胞衍生的体外 3D 器官模型,可在模拟内源性细胞组织和器官结构的环境中研究生物学过程,例如细胞行为、组织修复以及对药物或突变的反应。重要的是,3D 体外模型保留了体内肿瘤的组织病理学特征,包括患者特异性药物反应。类器官是一项重大技术突破,在药物筛选、疾病建模、基因编辑和移植...
Another, more technical points are the standardized and reproducible protocols for maintenance, culturing/seeding, cryopreservation and treatment of organoids, especially those enriched with various cell types [166]. Not only organoids are known to be heterogenous between the patients, but the addition ...
Chromosomal instability (CIN) is involved in about 70% of colorectal cancers (CRCs) and is associated with poor prognosis and drug resistance. From a clinical perspective, a better knowledge of these tumour’s biology will help to guide therapeutic strat