Organoids derived from epithelial tumors have recently been utilized as a preclinical model in basic and translational studies. This model is considered to represent the original tumor in terms of 3D structure, genetic and cellular heterogeneity, but not tumor microenvironment. In this study, we esta...
We used high-density chromosomal microarray analysis to evaluate CIN level of patient-derived organoids (PDOs) and their original mCRC tissues. We integrated the RNA-seq and mass spectrometry-based proteomics data from PDOs in a functional interaction network to identify the significantly dysregulated...
During the initial week, a Rho-associated kinase (ROCK) inhibitor was incorporated into the medium to promote organoid proliferation. Organoid culture The organoid medium was enriched with a combination of supplements, including B27, N-acetyl-l-cysteine, nicotinamide, human EGF, A83–01, human ...
After mechanical and enzymatic digestion, free cells have been seeded in Matrigel with proper medium. Development of organoids has been observed the day after seeding. Organoids have been passaged, expanded and stored in liquid nitrogen to constitute a biobank. After reaching appropriate volume, ...
Butyrate was recently shown to enhance the efficacy to radiotherapy in a patient derived organoid model [156]. Additionally, bacteria secreted SCFAs demonstrated modulation of immune responses. Fusobacterium nucleatum could drive a pro-inflammatory intestinal microenviroment through the SCFA receptor FFAR2,...
Butyrate was recently shown to enhance the efficacy to radiotherapy in a patient derived organoid model [156]. Additionally, bacteria secreted SCFAs demonstrated modulation of immune responses. Fusobacterium nucleatum could drive a pro-inflammatory intestinal microenviroment through the SCFA receptor FFAR2,...