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Total proteins were separated by SDS-PAGE gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, MA, USA), followed by blocking with 5% non-fat milk in Tris-buffer saline with 0.1% Tween 20 (TBST) for 1 h at room temperature. Then, membranes were ...
DE lncRNAs and circRNAs were identified through SDS RQ Manager 1.2 software (Applied Biosystems); normalization was performed using PPIA and GAPDH for tumor tissues and serum exosomes, respectively. miRNA expression was assessed through TaqMan microRNA assays and TaqMan Universal Master Mix II, no ...