作者基于活性调控区域由于缺乏组蛋白保护,因而更容易被酶切割的生物学假设,开发了一个工具(CRAG)来系统地在全基因组鉴定IFS(integrated fragmentation score)小的区域作为活性调控区域(cfDNA hotspots)。实验结果发现CRAG发现的区域主要富集到TSS...
We also provide another example which is based on the BH01 dataset (Snyder 2016), a deeply-sequenced cfDNA plasma sample. The fragment file can be downloaded athttps://zenodo.org/record/5227520. For you information, below are steps from the BAM file to the fragment file: ...