Exosome surface marker CD9 (Biolegend, clone: MZ3) and isotype control (Biolegend, #400511) were labeled on ice for 30 min, then washed and resuspend in FACS buffer and analyzed by BD™ LSR II flow cytometry and analyzed by the FlowJo® software. Absolute numbers of cells were ...
The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, ...
The “CRABP1 signalosome” theory prompted this current study. We exploited a combinatorial approach, starting with a mass spectrometry (MS)-based screening to identify all the possible binding partners of CRABP1, which was followed by bioinformatic analyses to uncover potential pathways or cellular p...
Apoptosis is a type of cell death which plays an important role in regulating growth, development, and immune responses1,2. Apoptosis is tightly controlled by multiple regulators, and the interaction between positive and negative regulators determines whether this program is activated or inhibited3. A...
We also determined whether Crabp1ARC neurons are sensitive to fasting, leptin, and GLP1R agonism by assessing cFOS immunoreactivity as a marker of neuronal activity. Results:Crabp1ARC neurons represent a novel GABAergic neuronal population robustly enriched in the ARC and are distinct from...
The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, ...