今天小编为大家带来一篇研究CPG甲基化特征的发表在10分+ Genome Biology的思路。题目为 CimpleG: fin ding simp le CpG methylation signatures。 数据介绍 本研究使用了来自GEO的以下数据集:GSE103253,GSE107226,GSE40699,GSE41933,GSE43976 等。此外将收集和策划的用于对细胞类型分类进行基准测试的数据集作为准备用...
CpG MTases, found in higher eukaryotes (e.g., Dnmt1), transfer a methyl group to the C5position of cytosine residues. Patterns of CpG methylation are heritable, tissue specific and correlate with gene expression. Consequently, CpG methylation has been postulated to play a role in differentiation...
Beyond this broad portrayal, there are vast gaps in our understanding of the pathway between neuronal activity and CpG methylation. These include the regulation in post-mitotic neurons of the executor proteins, such as the DNA methyltransferases, the elusive and putative demethylases, and the ...
Dynamic DNA methylation across diverse human cell lines and tissues. Genome Res. 2013 Mar;23(3):555-67. 但是,如果这个DNA的CpG岛被甲基化后,蛋白不能结合DNA,进而使转录沉默,基因不表达。 所以正因为CpG岛常位于基因转录调控区附近,与56%的人类基因组编码基因相关,所以基因转录区CpG岛的甲基化状态的研究...
发表于2017年,杂志是 JOURNAL OF CLINICAL ONCOLOGY影响因子26.303 , 文章是CpG Methylation Signature Predicts Recurrence in Early-Stage Hepatocellular Carcinoma: Results From a Multicenter Study亮点应该是自己的数据,然后使用了两个机器学习算法: LASSO, Least Absolute Shrinkage and Selector Operation; ...
each methyltransferase fragment is fused to zinc finger proteins with affinity for sequences flanking a targeted CpG site for methylation. We sought to improve the targeting of these enzymes by reducing the methyltransferase activity at non-targeted sites while maintaining high levels of activity at a...
参考文献: Lee, I., Razaghi, R., Gilpatrick, T. et al. Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing. Nat Methods (2020). https://doi.org/10.1038/s41592...
Ultra-high coverage that covering 27,000 CpG Islands, 2.03 million CpG sites, as well as 13 commercial methylation markers. Dual-strain dual-methyl status design for high performance and reduced cost. IGT® ssDNA Library Prep Kit enables low input amount samples like cfDNA. ...
CpG methylation, the most common epigenetic modification of vertebrate genomes, is primarily associated with transcriptional repression. MeCP2, MBD1, MBD2, MBD3 and MBD4 constitute a family of vertebrate proteins that share the methyl-CpG-binding domain (MBD). The MBD, consisting of about 70 ...
1. Feltus FA, Lee EK, Costello JF, Plass C, Vertino PM (2003) Predicting aberrant CpG island methylation, Proc Natl Acad Sci 100(21):12253−12258. 2. Jones PA, Baylin SB (2002) The fundamental role of epigenetic events in cancer, Nat Rev Genet 3(6):4z15−428. ...