一、CPEC组装的原理 CPEC组装是一种通过PCR实现DNA片段组装的技术。在这个过程中,DNA片段及载体经过变性解链,退火时末端同源序列互补,序列间互为模板和引物在DNA聚合酶的作用下延伸为含有缺口的环状DNA分子。这个缺口可以在大肠杆菌中得到修复,从而得到完整的...
Random mutagenesis, such as error-prone PCR (epPCR), is a technique capable of generating a wide variety of a single gene. However, epPCR can produce a large number of mutated gene variants, posing a challenge in ligating these mutated PCR products into plasmid vectors. Typically, the primer...
. I PCR is used to introduce overlapping regions with the vector or with adjacent ragments, primers should be designed to include at least two parts, each hybridizing to one end o the two neighboring rag- ments to be joined. I an additional short sequence needs to be ...
If PCR is used to introduce overlapping regions with the vector or with adjacent fragments, primers should be designed to include at least two parts, each hybridizing to one end of the two neighboring fragments to be joined. If an additional short sequence needs to be inserted between two ...
广藿香醇合酶倍半萜合酶环形聚合酶延伸克隆生物信息学目的 克隆广藿香中广藿香醇合酶(PTS)基因的cDNA序列,并构建其pRI101-PTS植物表达载体.方法 利用逆转录聚合酶链式反应(RT-PCR)获得PTS基因的cDNA序列,利用生物信息学软件对T-A克隆后的序列进行分析,预测其编码蛋白的理化性质,结构和功能.以环形聚合酶延伸克隆(...
人脉络丛细胞在大脑发育,成熟,衰老,内分泌调控和神经变性疾病的发病机理等多方面发挥作用。脉络丛由单层的上皮细胞组成,上皮细胞围绕血管中心共同构成血-脑脊液屏障。脉胳膜毛细血管是单层的被小孔阻隔的内皮细胞,小孔在内腔与胞间隙之间形成隔膜。研究表明,脉胳丛内皮细胞表达大量的Glut1葡萄糖转运蛋白。高密度的葡萄...
provided ready to use for a variety of analyses including: SNP analysis, DNA methylation analysis, Southern Blotting, and PCR. Genomic DNA from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues for isolation of DNA...
环境英语证书考试(PEC)-环境经济学词汇缩写 环境英语证书考试（PEC）-环境经济学词汇缩写 CFC: Chlorofluorocarbon CITES: Convention on International Trade in Endangered Species EETU: Environmental Education and Training Unit EEU: Environmental Economics Unit EIA: Environmental Impact Assessment ELIU: ...
SIU.PCR[ui16_GPIO_pin_no].R = 0x0500;eMIOS_0.MCR.B.GPRE = 0x81; //130/129+1 = 1MHzeMIOS_0_UC.UC[ui8_channel_no].A.R = 0;eMIOS_0_UC.UC[ui8_channel_no].B.R = 10;eMIOS_0_UC.UC[ui8_channel_no].C.B.EDPOL = 1;eMIOS_0_UC.UC[ui8_channel_no].C.B.MODE = ...
聚合酶链式反应可表示为 A.PEC B.PER C.PDR D.PCR 查看答案和解析>> 科目:高中生物 来源:2010年福建省厦门市第六中学高二下学期期中试题生物卷 题型:单选题 聚合酶链式反应可表示为 A.PEC B.PER C.PDR D.PCR 查看答案和解析>> 同步练习册答案全...