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The precleared protein was then incubated with 10 μl of Protein A/G PLUS-Agarose beads preincubated with the anti-β2AR antibody (Catalogue # sc-570, Santa Cruz, CA, USA) overnight at 4 °C with rotation. The beads were then precipitated by centrifugation and boiled with 2 × ...
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In order to check CRISPR knockout efficiency of GRHL3 IpA in keratinocytes and HCT116 cells, total genomic DNA was extracted using Quick-DNA Miniprep Plus Kit (Zymo). For sg-1 disruption, the corresponding genomic region harboring the knockout site was amplified using Phusion High-Fidelity DNA...
Click the plus symbol (+). By default, Mac OS X uses the Bonjour method to find the driver and add the product to the printer pop-up menu. This method is best in most situations. If Mac OS X cannot find the HP printer driver, an error message displays. Reinstall the software. ...