The RNA template-loop primer (5′-UUUUCUCCGCGUAGUUUUCUACGCG-3′) was annealed at 75 °C for 3 min and cooled to room temperature for 25 min in 1× ThermoPol buffer before adding the other ingredients. The final 20 µL mixture containing 5 µM of the RNA template-loop ...
Although their lipid core is a common feature of mRNA-LNPs, characteristics of the exact structure of this core and its dependence on lipid ingredients (molar ratios) and the localization of the mRNA are still under debate (see Fig. 3). mRNA is certainly located inside the LNPs, as determin...
Figure 7. Rapid antigen test (RAT) kit-based detection of SARS-CoV-2 infection: (1) test samples are collected in the form of swabs; (2) mixing the collected swab samples with buffer; (3) loading collected sample into the well of the antigen-coated strip; (4) formation of one band ...
Additional Methods: Details regarding the (1) enzyme inhibition assay, (2) fluorescence/luminescence interference test, and (3) colloidal aggregation study can be found in the Supporting Materials. 3. Results 3.1. Rationale for Screening HIV-1 Fusion Inhibitors as Possible Pan-CoV Inhibitors The Co...
Buffer II (Figure 1C) at either high or low RNA levels. Figure 1.Optimization of lysis buffers’ ingredients used in our in-house silica-column-based RNA isolation kits; (A) effect of ethanol concentration on RNA binding to silica columns; (B) comparison of Invitrogen Purelink kit with ...
Key ingredients and recycling strategy of personal protective equipment (PPE): Towards sustainable solution for the COVID-19 like pandemics. J. Environ. Chem. Eng. 2021, 9, 106284. [Google Scholar] [CrossRef] World Bank. Trends in Solid Waste Management. Available online: https://datatopics....
Ten microliters of specimen were loaded into the sample well of the device and three drops (~100 μL) of buffer solution were subsequently loaded into the sample well (Figure 2A). Results were interpreted within 15 min. The appearance of the control and test lines was assessed after 15 min...
First, it is important to conduct a usability test to evaluate whether people find the intervention platform easy to use and functional, as usability problems or ineffective systems might affect the magnitude of the treatment effect [58,59]. Additionally, the digital literacy of participants should...
Acinetobacter baumannii has been described as a cause of serious community-acquired infections in tropical countries. Currently, its implications when simultaneously identified with other pathogens are not yet adequately understood. A descriptive study w
known hypersensitivity to any STX ingredients and its metabolites, cognitive impairment, use of a high-flow nasal cannula or non-invasive ventilation, alcohol or substance abuse, known pregnancy, breastfeeding, or ongoing or prior participation in other clinical trials. The full list of inclusion and...