For PNT, samples are considered positive with values below a threshold set as the mean minus threefold the standard deviation determined on a collection of pre-pandemic sera assuming a normal distribution (Shapiro–Wilk normality test W = 0.9943, p = ns). This threshold permits ...
. Results are expressed as percent neutralization in a presence of respective mAb relative to control wells with no CPE minus CI values from control wells with maximum CPE. RTCA IC50 values were determined by nonlinear regression analysis using Prism software. SARS-CoV-2 VSV-G virus production ...
Minus-strand copies of replicating coronavirus mRNAs contain antileaders J. Virol., 65 (1) (1991), pp. 320-325, 10.1128/JVI.65.1.320-325.1991 View in ScopusGoogle Scholar [78] J. Krijnse-Locker, M. Ericsson, P.J.M. Rottier, G. Griffiths Characterization of the budding compartment of ...
The extracted and purified genomic RNA was transcribed to cDNA using Maxima H Minus Double-Stranded cDNA Synthesis Kit, catalog number K2561 (ThermoFisher Scientific, Waltham, USA) following the manufacturer's in- structions. Library preparation was achieved using Nextera™ Flex for Enrichment ...
(mean spot count of technical replicates normalized to 500,000 cells minus the respective negative control) (a). The number of CoVac-1 T cell epitopes (n = 6) per participant that elicited a vaccine-induced T cell response (b). Intensities of CoVac-1-induced IFNγ T cell ...
PR outcomes were calculated as the post-rehabilitation value minus the pre-rehabilitation value. The threshold for statistical significance was set at an alpha level of 0.05. All statistical analyses were performed using SAS software, version 9.4. Patient demographics We identified 55 patients diagnosed...
CalREDIE episode days and months before birth were calculated as the maternal episode date minus the infant’s birthdate. We retained linkages that occurred both before and after birth for analysis. Negative episode dates occurred prior to birth and those identified at 0 days on the date of de...
The minus one (−1) ribosomal frameshifting just upstream of the stop codon in the overlapping region between ORF1a and ORF1b ends up in bypassing the ORF1a stop codon. This directs the process into the production of the larger pp1ab polyprotein [2,17,19]. The presence of a hairpin...
X,Y,Z are random variables with finite variances. Prove {eq}Cov(X, Y) = E[Cov(X, Y|Z)] + Cov(E[X|Z), E[Y|Z]) {/eq} Finite and Infinite variance: These are those components of variance to identify the tail index in order to determine the...
1b and Additional file 5) but did not correlate with CRP levels (Additional file 6). Next, we analysed the relationship between baseline plasma levels of inflammation markers and organ failure worsening between days 0 and 3–4. Organ failure worsening, defined as an increase of SOFA score ...