Data dependent MS2 spec- tra of precursor ions were acquired with a resolution of 17,500 FWHM, an AGC target of 5 × 105, 100 ms MIT and a normalized collision energy of 30. An exemplary extracted
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of the main regulatory epigenetic mechanisms51. This modification, in most cases, impairs transcription factor binding in the CpG-rich promoter region of a gene52, thereby leading to lower transcription. However, it still remains elusive why susceptibility to environmental cues is especially high duri...
The mRNA populations were quantified by spec- trophotometric measurements at 260 nm. They were then analyzed for quality by denaturing agarose gel electropho- resis and probing of Northern blots with the housekeeping gene elongation factor 1-α. Construction of SSH libraries was performed by means ...
of the main regulatory epigenetic mechanisms51. This modification, in most cases, impairs transcription factor binding in the CpG-rich promoter region of a gene52, thereby leading to lower transcription. However, it still remains elusive why susceptibility to environmental cues is especially high ...
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