Flow Cytometry Compensation Beads Flow cytometry provides a rapid method to quantify cell characteristics. However, most flow cytometers cannot directly provide the cell concentration or absolute count of cells in a sample. Absolute cell counts have been widely use...
A mixture of live and heat-killed Jurkat cells (human T-cell leukemia) were treated with the reagents in the LIVE/DEAD(R) Viability/Cytotoxicity Kit (L3224), counting beads were added and the sample analyzed by flow cytometry using 488 nm excitation.Quick Facts...
The BD Accuri C6 flow cytometer has fixed alignment and pre-optimised detector settings, can record volume of sample processed without use of counting beads, and is small enough to fit on a benchtop or inside a bio-containment hood. SYBR-Gold (SG), a proprietary cyanine dye (excitation ~...
BD Biosciences is a leader in the use of flow cytometry for determining immune system status and for counting CD4 cells in patients with human immunodeficiency virus (HIV) infection. The company has gained this position through many years of basic research and product development in immunology and...
We compared nuclei counts by flow cytometry with cell DNA analysis, using two commonly used fluorescent dyes. With CyQUANT, we obtained 6.4–6.5 pg of DNA per cell for rat islets and 6.9 pg of DNA per cell for human islets. These values are comparable to estimates for human cells (6.6 ...
CountBright ™ Absolute Counting Beads * for flow cytometryMolecular Probes