Gram Stain | Purpose, Theory & Procedure from Chapter 4 / Lesson 3 75K Learn how to perform a Gram stain, and see the purpose of Gram staining. Know Gram stain steps, and a description of what happens at each step in the Gram stain. Related...
Expression of the GFP fusions was induced by addition of 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) in the media. Cells were observed by Axio Imager A2 fluorescence microscopy (Carl Zeiss, Oberkochen, Germany) after 30 min of staining in Hoechst33258, which dyes DNA specifically, ...