Unfortunately, many new genome projects lack comprehensive experimental data to derive a reliable initial set of genes. RESULTS: In this study, we report a computational method, CEGMA (Core Eukaryotic Genes Mapping Approach), for building a highly reliable set of gene annotations in the absence of...
Enhancers and eukaryotic gene transcription These properties had set them apart from previously described upstream promoter elements of eukaryotic genes. More recent investigations suggest that enhancer and promoter elements overlap both physically and functionally. Our current view is ... E Serfling,M Jas...
As DNA topo II influences histone deposition and chromatin compaction and is the second most abundant nuclear protein after histones, we suggest MV genes underlie a proto-chromatinized replisome that diverged prior to diversification of eukaryotic core histone variants. Thus, combined domain architecture...
Here, FANCL, Ube2T, and FANCM were identified as important HR-promoting factors using an unbiased genetic screening approach targeting 2760 genes. Similarly, a recently published screen that studied HR with an ectopically provided dsDNA donor, also identified FA genes to be strong drivers of this ...
The nucleosome is one of the hallmarks of eukaryotes, a dynamic platform that supports many critical functions in eukaryotic cells. Here, we engineer the in vivo assembly of the nucleosome core in the model bacterium Escherichia coli. We show that bacter
Much evidence has now emerged showing that epigenetic factors also contribute to transcriptional control of eukaryotic genes [3]. The term epigenetic has been redefined in a modern context as "the structural adaptation of chromosomal regions so as to register, signal or perpetuate altered activity sta...
713 reads per sample and a 94.6% mapping rate to the maize reference genome (B73-NAM-5.0). Compared to the control groups treated with sterile water, we identified 293, 387, and 171 differentially expressed genes (DEGs, |log2(fold-change) |> 1,Padj < 0.05) in maize roots ...
NetAct also has a unique approach to infer the TF activity from the gene expression of the target genes with the consideration of activation/inhibition nature. From our in silico benchmark tests, we found that NetAct outperforms major activity inference methods, owing to the design of the filt...
(i.e., the map with the lowest p value). atm/atr regulates both the checkpoints of the g1/s and s/g2. dna damage checkpoints pathways arrest or delay the progression of cell cycle in response to the dna damage. eukaryotic cell cycle have four phases, g1 (g indicating gap), s ...
[30], 40,122 forAmphimedon queenslandica[19], and 37,416 forTethya wilhelma[29]. This observation is validated by the high number of conserved core eukaryotic genes identified in theO. minutadraft sequence (91 to 93% using BUSCO (Benchmarking Universal Single-Copy Orthologs) [64]) attesting...