Copper homeostasis in Gram-positive bacteria essentially requires three components: a copper exporting ATPase (founding member CopA), a copper chaperone (founding member CopZ), and a copper-responsive regulator (founding member CopY) which regulates the expression of these functions. GSH works as a ...
Although the anticancer mechanism of elesclomol is still controversial, there is no doubt that the anticancer effect of elesclomol depends on the existence of copper ions in the external cellular environment [6,7]. Extracellularly, elesclomol can form a stable 1:1 complex with Cu(II) [54]...
Hepatic GPI-CP expression was attenuated in a murine model of Wilson's Disease (which results from mutations in the ATP7B copper-transporting ATPase), possibly contributing to the noted iron accumulation in hepatocytes and Kupffer cells, which resulted in a functional iron deficiency (Jonczy et ...
Copper ions induce expression of the Bacillus subtilis copZA operon encoding a metallochaperone, CopZ, and a CPx-type ATPase efflux protein, CopA. The copZ... Gaballa,A. - 《Microbiology》 被引量: 42发表: 2003年 How cells handle copper: A view from microbes Copper is an essential elemen...
The interaction of Cu-ATOX1 with these sites (preferentially with MBDs 1 to 4) induces conformational changes and activates ATPase catalytic activity. Other highly conserved domains include the nucleotide-binding domain (N-domain) for ATPase catalytic activity, the P-domain for phosphorylation, and ...
(Atox1) is cytosolic Cu chaperone that obtains Cu through the Cu importer CTR1, and transports Cu to the Cu-transporting/exporting ATPase (ATP7A) to maintain the intercellular Cu homeostasis16,17. In addition, Atox1 delivers Cu through ATP7A to the secretory Cu-dependent enzymes such as ...
As mentioned above, mutations in the ATP7B gene, which encodes the Cu-transporting P-type ATPase ATP7B, are associated with Wilson’s disease, a life-threatening disorder in which patients present with progressive Cu accumulation in several tissues, particularly the liver, brain, and cornea. Impo...
We have shown that the open reading frame ybbI in the genomic sequence of Escherichia coli K-12 encodes the regulator of expression of the copper-exporting ATPase, CopA. In vivo studies showed that ybbI (designated cueR for copper export regulator gene) was required for copper tolerance during...
Unlike Cu+ transport by the P-type ATPase Ccc2 discussed below, Ctr activity is not dependent on ATP hydrolysis, thus the mechanistic understanding of how Cu+ traverses through the Ctr pore remains to be fully deciphered. However, recent insights into the 3D structure of human Ctr1 revealed...
The present application relates to a novel copper-exporting P-type ATPase A variant, an Escherichia coli strain comprising the variant, and a method for producing L-tryptophan using the strain.PARK, GOUNCHANG, JIN SOOKJUNG, MOO YOUNGKIM, HYUN AH...