ApplicationDilution Western Blot (WB)WB : 1:500-1:1000 Immunohistochemistry (IHC)IHC : 1:50-1:500 It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery. ...
产品描述 概述 E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involve...
Application of PD-1 blockade in Cancer immunotherapy. Comput Struct Biotechnol J. 2019;17:661–74. Article CAS PubMed PubMed Central Google Scholar Borghaei H, Paz-Ares L, Horn L, Spigel DR, Steins M, Ready NE, et al. Nivolumab versus docetaxel in advanced nonsquamous non-small-cell ...
Foliar application of thiourea, salicylic acid, and kinetin alleviate salinity stress in maize grown under etiolated and de-etiolated conditions Granaz Kanval Shaukat Abdul Wahid Discover Food (2022) Guard cells control hypocotyl elongation through HXK1, HY5, and PIF4 Gilor Kelly Danja Brandsm...
SPAs interact with COP1 to form a tetramer to enhance the activity of COP1 to ubiquitinate target proteins [34]. MpCOP1 (Mg5g12010) is the ortholog of AtCOP1 in the genome of M. polymorpha that also contains a RING-finger domain in the N-terminal binding to ubiquitin conjugating enzyme...
The application of E64 also elevated BES1 abundance, but contrary to MG132, the effects of cop1 and E64 were additive (Figure S7). Consistently with the notion that the effects of PIF4 on nuclear BES1 abundance are largely accounted for by changes in BES1 expression, the positive effects...
“Improved Sensitivity ofNucleic Acid Database Search Using Application-Specific Scoring Matrices”MethodsA companion to Methods in Enzymology 3(1)66-77;StevenHenikoff and Jorja G.Henikoff.1992“Amino acid substitution matrices fromprotein blocks.”Proc.Natl.Acad.Sci.USA.89(biochemistry)10915-10919;M....