Precursor NEDD8 is processed at the C-terminal to the activated form by DEN1. NEDD8 is conjugated to the target substrate through an E1 (NAE), and E2 (UBE2F or UBE2M), and an E3 (shown are RBX1/2, RNF111 and DCUN1D members). Deneddylation is achieved by the CSN and DEN1. The...