Cells were lysed in the same buffer as for western blot analyses and lysates were separated on a Superose 6 10/300 GL column (GE Healthcare, Little Chalfont, UK). Fractions of 1 ml were collected and precipitated with deoxycholic acid and trichloroacetic acid. The resulting pellets were ...
The Institution Animal Care and Use Committee approved the experimental protocols. 3.2. Cell Culture The medium was a 1:1 mixture of DXOOEHFFR¶V PRGLILHG (DJOH¶V PHGLXP DQG +DP¶V QXWULHQW mixture F12 (DMEM/F12, Sigma, St. Louis, MO, USA) supplemented with heat-...