(pH 8.0), 50 mM of KCl, 2 mM of MgCl (2), 0.2 mM of each of four deoxynucleotide triphosphates, 1 unit of DNA polymerase (EuroTaq; Euroclone, Paignton, UK), and 100 ng of genomic DNA using 0.4 mM of each of a primer pair of 5'-GGAAAGTAGAACATACATTAAATG-3' and 5'-CCTG- ...
number of clinical and biochemical factors except that the "abnormal responders" had a significantly (p less than 0.03) greater control AII level (37 +/- 3 vs 29 +/- 3 pg/ml) and lower control plasma aldosterone level (14 +/- 2 vs 22 +/- 3 ng/dl) than the "normal responders."...
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Plasma renin concentration was not significantly increased in the untreated infarct group (155.4 +/- 136.7 ng AI/ml/hr) as compared to the sham-operated group (47.6 +/- 15.9 ng AI/ml/hr). Plasma aldosterone did not change in the three experimental groups. The plasma level of immunoreactive...
participants with P-HPT had a decrease in systolic blood pressure (SBP) (−6.4 mmHg, P < 0.01), an increase in plasma renin activity (PRA) (+1.50 ng/mL/h, P = 0.06), and a decrease in PTH (79.5 (21.6) to 70.9 (19.6) pg/mL, ∆ = −8.6 pg/mL, P = 0.049);...
Angiotensin-converting enzyme gene polymorphism predicts the time-course of blood pressure response to angiotensin converting enzyme inhibition in the AASK trial. J Hypertens 2007; 25: 2082–2092. 53 Busquets X, MacFarlane NG, Heine-Suner D, Morla M, Torres-Juan L, Iglesias A et al. ...
To determine whether the furin convertase is coordinately regulated with TGF-β1, the NRK cells were incubated in the presence of 5 ng/ml of TGF-β1 or 10 μmol/L PMA and mRNA analyzed for specific TGF-β1 and furin messages. These stimuli were chosen because they are well established ...
Hello everyone! I'm working on a flow that takes the response from an MS Form, imports the response into an HTML document, converts the HTML to a PDF,...
showed that blocking ANG II activity through the AT1R (angioten- sin type I receptor) blocker losartan prevented lung fibrosis in response to hyperoxia. The role of ACE-2 in these models, however, has not yet been evaluated. The function of ACE-2 as the cellular receptor for SARS- ...
To prevent shredding of the DNA, the sample was not vortexed. The final gDNA concentration was 500 ng/ml as determined by optical density at 260 nm. cDNA library construction and sequencing. P. stipitis CBS 6054 was grown at 30 1C in 200 ml of either YPD or YPX in either a 2.8 l ...