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SNA lectin recognizes Neu5Ac (a2–6)Gal/GalNAc residue found in N-linked and O-linked glycans36,37; however, the removal of N-linked glycans by PNGase F treatment selectively abolished SNA binding to the Fc fragment, but not to the Fab fragment even under SDS denaturation (Supplementary ...
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heating at 100 °C for 10 min in presence of 1% SDS. Denatured IgGs were neutralized by Nonidet P-40 and incubated with or without 1 unit per μl PNGase F (Roche) for 24 h at 37 °C. Then, PNGase F-treated IgG was subjected to western or lectin blotting as described ...